To explore the effects of Ts-EVs on the local T-cell immune response during TNBS-induced colitis, the levels of mRNA coding for T helper- and Treg-associated cytokines such as IFN-γ, IL-4, IL-17A, IL-10, as well as for the transcription factors T-bet, GATA-3, ROR-γt, Foxp3 were analyzed by qRT-PCR after extracting total mRNA from colon tissue. This evidence concerns the gene IL4 and colitis.