NEFL and ischemic stroke: Plasma NF-L levels were significantly increased in ischemic stroke patients acutely (<8 h) (28.70 pg/mL; IQR 15.20–68.30) and at 72 h (31.70 pg/mL; IQR 16.70–98.40) compared to healthy controls (14.10 pg/mL; IQR 7.73–18.96) (Kruskal–Wallis test: chi square = 18.01, p = 0.0004, followed by Dunn's multiple comparisons test: p < 0.01) (Figure 2).