However, as both Nec-1 and SB216763 reduce infarct size in the acute stage of ischemia in animals subjected to tMCAO- or pMCAO-induced injury (Valerio et al., 2011; Chen et al., 2018b; Ni et al., 2018; Wang et al., 2019), we cannot exclude the potential contribution of Nec-1 or SB216763 in mediating a reduction in infarct size. Here, PCSK1 is linked to ischemia.