These data indicate that SB216763 and Nec-1 inhibit inflammatory cytokine production by blocking RIP1K and GSK3β activation, and that these two kinases may interact during the formation of ischemic stroke-induced glial scars; the combination of lower dose of Nec-1 (10 uM) and SB216763 (1 uM) might produce synergistic pharmacological effects in reducing the glial scar formation. Here, PCSK1 is linked to ischemic stroke.