To evaluate the NF-L levels in different neurodegenerative disease mouse models, NF-L was quantified in Line 61 mice, 5xFAD mice, TAR6/6, and SOD1-G93A low expressor mice as well as 4L/PS-NA mice as model of Parkinson’s disease, AD, ALS, and GD, respectively. Here, NEFL is linked to neurodegenerative disease.