For these studies, we incorporated a novel vaccination regimen in which one could achieve high levels of CD8 T-cell responses using antigen-loaded DCs followed by peptide-based vaccine (TriVax) booster immunisation.11 Notably, PLK1122DC_TriVax regimen could be applied to various tumour-types to provoke tumour regression and prolonged survival, and tumour re-challenges in mice that rejected their initial tumours verified the presence of long-term antigen-specific CD8 T-cell memory immunity capable of rejecting even other types of tumour rechallenge (Fig. S3). The gene discussed is CD8A; the disease is neoplasm.