So, in the present study, through integrated analysis of TARGET and GEO datasets, we identified eight MYCN amplification associated genes ARMC6, DCTPP1, EIF4G1, ELOVL6, FBL, PRMT1, E2F1 and RPS19 which had significant prognostic effects in pediatric neuroblastoma patients. The gene discussed is E2F1; the disease is neuroblastoma.