To monitor the position and morphology of human cancer cells in the CAM-Delam assay, four different cancer cell lines; colon (SW620), prostate (PC-3U), lung (A549) and glioblastoma (U251), were engineered using the CRISPR/Cas9 technique24,25 to carry a GFP expressing transgene in the AAVS1 locus (Fig. S2). The gene discussed is PPP1R12C; the disease is cancer.