To measure compound effect on mTORC1 activity in tauopathy A152T neurons, we treated cells for 24 h and measured the levels of mTORC1 substrates phosphorylation (Fig. 6e), including P-mTORS2448, which can result from mTOR auto-phosphorylation or p70S6K activity feedback-loop64 (Fig. 6a). The gene discussed is RPS6KB1; the disease is tauopathy.