For this, we injected 5A2-DOT-10 LNPs encapsulating Cas9/sgDMD RNPs into Tibialis Anterior (TA) muscles of ΔEx44 DMD mice weekly (three injections, 1 mg kg−1 sgRNA) and detected the expression of dystrophin protein in TA muscles 3 weeks after the last injection (Fig. 5a). The gene discussed is DMD; the disease is Duchenne muscular dystrophy.