NOTCH1 and neoplasm: In addition, we designed two shRNAs targeting endogenous Notch1 (the N terminus of Notch1, shRNA-N) or pan-Notch1, including both SB transposon-driven Notch1 and endogenous Notch1 (the C terminus of Notch1, shRNA-ICN) (Supplementary Fig. 4a, b), and applied them to cell lines derived from Notch1-driven SB tumours (MK1370-3R) and cell lines derived from non-Notch1-driven SB tumours (MK1097-5R) (Fig. 3e, Supplementary Fig. 4c, d).