To further clarify the effect of APN on the expression levels of AMPK, PI3K, and Akt in PCOS mouse uterine tissue, Western blot was used to analyze the protein expressions of phosphorylated AMPK (p-AMPK), phosphorylated PI3K (p-PI3K), and phosphorylated Akt (p-Akt). The gene discussed is AKT1; the disease is polycystic ovary syndrome.