CYP3A4 and cholestasis: In agreement with the data of Behr et al. (2020b), we found, besides the downregulation of CYP7A1, also an upregulation of CYP3A4, which both have been described as key events in the AOP for cholestasis, and, thus, may contribute to the development of cholestasis (Vinken et al. 2013).