To examine gene expression changes in AMs during AAD driven by a clinically-relevant aeroallergen, mice were repeatedly exposed to inhaled HDM extract for 3 weeks (Figure 1A), a model shown previously in our laboratory to induce the hallmark features of allergic asthma, including elevated serum IgE, airway hyperresponsiveness, production of type 2 cytokines and eosinophilic pulmonary inflammation33. The gene discussed is IGHE; the disease is airway hyperresponsiveness.