ANXA5 and acute lymphoblastic leukemia: To confirm that the loss of cell viability is due to the cytotoxic effect of I-CRP and not due to a metabolic effect, we used a cell death assay analyzing phosphatidylserine (PS) exposure (annexin-V) and membrane permeabilization (propidium iodide, PI) at different concentrations of I-CRP (0.4, 0.6, 0.8, and 1.0 U/mL), after 24 and 48 hours of treatment (Figure 2) in T-ALL cells and the healthy counterpart.