In our study, p65 positively regulate FPGS transcription, and after inhibition of NF-κB activity by PDTC in Jurkat cells, the mRNA level of anti-apoptotic gene Bcl2 increased (Additional file 4: Figure S3), implicating a pro-apoptotic role of NF-κB in the MTX induced apoptosis in leukemia cells. The gene discussed is BCL2; the disease is leukemia.