To interfere with β-catenin/TCF functionality in Wnt-active CRC cell lines, we stably transduced LS174T and SW480 to express the CTNNB1-binding domain of TCF7L2 (nTCF: amino acids 1 to 90) fused to a tamoxifen-inducible version of the hormone-binding domain of the estrogen receptor (ERT2) (here referred to as nTCF-ERT2 or, as a cell line suffix, −NE). Here, HNF4A is linked to colorectal carcinoma.