LARGE2 and metastatic neoplasm: Similarly, RNA-Seq data derived from the TCGA-COAD cohort [46] did not show differential expression of LARGE2 between M0 (n = 365) and M1 (n = 71) tumors (Additional file 10), suggesting that the elevated LARGE2 gene expression level found in CRC cohorts and PDTOs is not affected by metastatic disease progression.