To address the relevance of LARGE2 for functional α-DG O-glycosylation in CRC, we generated targeted knock-outs (KOs) of LARGE2 in CRC cell lines and a patient-derived tumor organoid (PDTO) line PDTO1 (Additional file 5) by CRISPR/eCas9-mediated genome editing using two guide RNAs directed against different regions of the LARGE2 open reading frame (Fig. 3A, upper panel). The gene discussed is LARGE2; the disease is neoplasm.