LARGE2 and colorectal carcinoma: Furthermore, CRISPR/eCas9-mediated targeting of the TCF7L2-BS within LARGE2 in SW480 and in the CRC organoid line PDTO1 (Additional file 5), which led to decreased LARGE2 mRNA levels (Fig. 1I and Additional file 6I), compromised O-glycosylation of α-DG (Fig. 4H,I).