In the present study, FAM83H‐AS1 expression level was higher in TGF‐β‐treated Eca109 cells than control cells, and high level of FAM83H‐AS1 resulted in the up‐regulation of EMT‐initiating transcriptional factors and mesenchymal markers, and the down‐regulation of epithelial markers at mRNA and protein levels, suggesting that FAM83H‐AS1 expression was induced by TGF‐β and promoted EMT in oesophageal cancer metastasis. The gene discussed is TGFB1; the disease is carcinoma of esophagus.