In the present study, we aimed at providing an integrated analysis on the expression and correlation between FAM83H‐AS1 and FAM83H, the potential biological function of these antisense‐sense strands and downstream regulatory mechanism of FAM83H‐AS1 in the pathogenesis of ESCC, as well as its role in TGF‐β‐induced EMT. Here, TGFB1 is linked to esophageal squamous cell carcinoma.