TFRC and Splenomegaly: Low spleen CsA levels in the P2Ns-GA-CsA group, on the other hand, may be explained by a multitude of factors, including the absence of afferent lymphatic vessels to the spleen, changes in drug retention due to different severity of splenomegaly among the treatment groups, changes in the activity of reticuloendothelial system (27), and increased drug uptake by nonspleen tissues in the P2Ns-GA-CsA group as a consequence of CD71-mediated active delivery.