UNC13D and infection: Peripheral blood mononuclear cells were isolated from healthy volunteers or FHL3 patients, stimulated for 2 days with T cell agonists, and transduced at a multiplicity of infection (MOI) of 100 with either a VSVG-lentiviral vector (LV) encoding GFP or human Munc13-4 isoforms C-terminally tagged with GFP (Figure 5A).