Our results support the hypothesis that repeated CSF-470 plus BCG, and GM-CSF vaccination may have caused waves of tumor Ags release from the irradiated vaccine cells, and subsequently, Ag-stimulated CD8+ and CD4+ T cells of the host may have lysed micro-disseminated tumor cells, leading to repetitive endogenous re-stimulation events broadening the immune response. This evidence concerns the gene CD4 and neoplasm.