Third, hyperglycemia-induced ROS and methylglyoxal production has shown to regulate expression of RAGE, S100A8, S100A12, and HMGB1 expression, which was normalized by overexpression of mitochondrial uncoupling protein 1, superoxide dismutase 2, or glyoxalase I. Loss of GLO1 mimicked the effect of high glucose whereas overexpression of GLO1 normalized the increased binding of NFκB p65 and activator protein 1 to the respective promoters (100), which might be mediated by SET7 (101). This evidence concerns the gene HMGB1 and Hyperglycemia.