Furthermore, the expression of enzymes (PEMT, LCAT, PTDSS1, PTDSS2, and PLA2) involved in the glycerophospholipid metabolism of the liver was changed in NAFLD model rats and nuciferine treatment regulated the expression of PEMT, LCAT, PTDSS2 and PLA2. PC could be synthesized from choline or be transformed from PE under the catalysis of phosphatidylethanolamine N-methyltransferase (PEMT). The gene discussed is PTDSS2; the disease is metabolic dysfunction-associated steatotic liver disease.