Furthermore, the expression of enzymes (PEMT, LCAT, PTDSS1, PTDSS2, and PLA2) involved in the glycerophospholipid metabolism of the liver was changed in NAFLD model rats and nuciferine treatment regulated the expression of PEMT, LCAT, PTDSS2 and PLA2. PC could be synthesized from choline or be transformed from PE under the catalysis of phosphatidylethanolamine N-methyltransferase (PEMT). This evidence concerns the gene LCAT and metabolic dysfunction-associated steatotic liver disease.