To investigate whether the protective effects of NBP against demyelination and cognitive dysfunction following CCH involve the suppression of inflammation, we measured the protein levels of TNF-α, p-STAT3, and NF-κB by western blotting and the mRNA level of TNF-α by qRT-PCR (Figures 6A–E). The gene discussed is NFKB1; the disease is columnar cell hyperplasia of the breast.