ERG and posterior cortical atrophy: We treated the VCaP PCa cell line with a single dose of either the E43′ or E45′ SSOs and observed that both SSOs could cause skipping of exon 4 in ERG mRNA as assessed by reverse-transcription PCR (RT-PCR) from as early as 24 h up to 72 h following dosing (Fig. 1b, c).