Notably, among 30 differentially methylated PcG target developmental genes, 29 (e.g., HOXA6, HOXB3, and MEIS1) were hypomethylated in DNMT3A mutant AML (R882: major mutation) relative to DNMT3A WT AML, suggesting that inactivating DNMT3A mutation causes defects in epigenetic repression of PcG target developmental genes (Fig. 6c, d). Here, DNMT3A is linked to acute myeloid leukemia.