To confirm this result, samples were also harvested for Western blot analysis following 18 h of infection with the same four strains of Toxoplasma. Lysates of these samples were resolved by gel electrophoresis, blotted, and stained with the Y69 antibody recognizing c-Myc; the results (Fig. 2B) showed that RH and the complemented RHΔgra16::GRA16HA strain induce robust c-Myc expression, while the GRA16 deletion eliminates almost all of this upregulation. Here, MYC is linked to infection.