To confirm that the regulatory effects of Bmi-1 on RKIP are mediated through miR-27a and miR-155, we introduced miR-27a inhibitor and miR-155 inhibitor to GC cells with ectopic expression of Bmi-1 and miR-27a mimic and miR-155 mimic to GC cells with Bmi-1 gene knockdown. The gene discussed is BMI1; the disease is gastric cancer.