SPARC is frequently methylated in lung cancer and not in our panel of normal samples tested; moreover, SPARC methylation can be easily detected in FFPE tissues by QMSP methodology and this molecular approach was able to rapidly assess the global methylation of 11 CpGs located into the main CpG island of the gene [14,31] with a sensitivity of 61% and a specificity of 100%. This evidence concerns the gene SPARC and lung cancer.