Variable levels of methylation of SPARC were observed in the tumor cell lines ranged as follows: 0–292.5 ± 60.6 (A549), 23.4 ± 7.4 (H2228), 148 ± 6.8 (H1573) and 179.3 ± 20.4 (H460), (Figure 4A), whereas in normal cells no methylation was detected. Here, SPARC is linked to neoplasm.