DNA NC nanoparticles loaded with RNP (ribonucleoproteins such as Cas9) targeting the EGFP gene when administered intratumorally to U2OS.EGFP tumor xenografted mice led to a 25% reduction in EGFP gene expression in U2OS.EGFP tumor cells after 10 days [142]. The gene discussed is RNPC3; the disease is neoplasm.