As metabolic disorders such as cardiovascular disease and obesity are associated with high levels of inflammatory molecules and elevated levels of cholesterol, we sought to determine the relationship between the gene expression levels of the cytokines IL6 and TNFα in different adipose depots and the serum total cholesterol levels of chow (C)- and high-fat (HF)-fed mice compared to the percentage change in adipocyte volume in gWAT (Supplementary Figure S5A–C), iWAT (Supplementary Figure S5D–F) and PAT (Supplementary Figure S5G–I). This evidence concerns the gene TNF and metabolic disease.