As obesity, induced by a state of high caloric intake, is associated with chronic inflammation, we next examined whether the pro-inflammatory cytokines IL6 (Figure 4A) and TNFα levels (Figure 4B) were altered in by HF diet in gWAT, iWAT, iBAT and PAT compared to their chow-fed counterparts. The gene discussed is TNF; the disease is obesity due to melanocortin 4 receptor deficiency.