Herein, we provide a new evidence that the cardiomyocyte‐specific overexpression of FNTB induces cardiac hypertrophy in cultured primary rat neonatal cardiomyocytes, characterized with an increase in the size of cells and the levels of hypertrophy biomarkers ANP, BNP and β‐MHC at the mRNA level and NPPA at the protein level. Here, NPPB is linked to cardiac hypertrophy.