CD8A and melanoma: Isolated splenocytes were stimulated with mTRP1181–188 or mTRP2180–188 (i.e., H-2b-restricted immunogenic epitopes of melanoma antigens) in mixed lymphocyte cultures (MLCs) for 5 days to allow selective proliferation of the respective antigen-specific CD8 T cells and thus increase the chance of their detection via multimer staining.