In the top 10 up-regulated genes, S100P and SPP1 were highly expressed in HCC tissues compared with normal liver tissues, whereas PLA2G2A and APOA4 expression were down-regulated in HCC (Fig. 2b); highly expressed SPP1, TMEM92, and EGLN3 predicted a poor prognosis in HCC patients (Fig. 2c and Supplementary Fig. 1). This evidence concerns the gene TMEM92 and hepatocellular carcinoma.