Data showed that expression of TRIM25 was lower in GC tissues than in adjacent normal tissues (P = 0.0029, Fig. 6d); in contrast, expression of SP1 was higher in GC tissues than that in adjacent normal tissues (P < 0.0001, Fig. 6e); and the SP1 protein level was inversely correlated with the TRIM25 in primary tumors from GC patients (r = − 0.255, P = 0.0153, Fig. 6f, Additional files 13: Table S5). Here, TRIM25 is linked to gastric cancer.