Higher age, GGT, ALT, AST, TP, ALB, GLB, TB, DBIL, BUN, Cr, UA, FPG, TC, TG, LDL-c, BMI and AIP were found in patients with than without NAFLD, thus indicating that these variables are risk factors in the progress of fatty liver disease. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.