To assess the role of beclin-1 in GKB-induced autophagy, we examined the expression levels of p62 and LC3B (which is involved in autophagy) in beclin-1 knockdown lung cancer cells and found that p62 and LC3B (GKB-induced autophagy) were decreased in the beclin-1 knockdown group compared to the control group (Fig. 6b). The gene discussed is BECN1; the disease is lung cancer.