To address this, we incorporated Ascl1GFP knock‐in (null) and Ascl1Floxed alleles into the glioma mouse model to generate GlastCreERT2;Ascl1GFP/F;Nf1F/F;Tp53F/F and GlastCreERT2;Ascl1F/F;Nf1F/F;Tp53F/F mice, respectively, both of which when administered with tamoxifen at E14.5 will result in triple conditional knock‐out of Ascl1 along with Nf1 and Tp53 (Ascl1;Nf1;Tp53CKO). Here, TP53 is linked to central nervous system cancer.