ChIP‐seq for ASCL1 has previously been performed for glioma cell lines in culture revealing that ASCL1 directly interacts with Wnt signaling by binding to genes such as AXIN2, DKK1, FZD5, LGR5, LRP5, TCF7, and TCF7L1. A model was proposed in which ASCL1 functions at least in part by repressing an inhibitor of Wnt signaling, DKK1, resulting in increased signaling through this pathway to maintain the tumorigenicity of glioma cells (Rheinbay et al., 2013). This evidence concerns the gene LRP5 and central nervous system cancer.