In this study, we found EBNA1 increased infiltration of Tregs in tumor microenvironment through converting naïve T cell into Tregs via upregulate TGF‐β1, upregulated CCL20 increased Tregs migration, and polarized‐M2 macrophage converted naïve T cells into Tregs. The gene discussed is TGFB1; the disease is neoplasm.