This resulted in a significant increase in cilia percentage on duct-like cysts marked with SOX9, from 4±1.2% (n=402 cells from four biological replicates) in DMSO-treated KC ADM cells to 26.7±3.2% (n=587 cells from four biological replicates, P<0.0001) in MLN8237-treated KC ADM cells cultured for 7 days (Fig. 5C-F). This evidence concerns the gene SOX9 and keratoconus.