To explore the roles of PSPC1 in cell motility, we selected three human hepatocellular carcinoma (HCC) cell lines including SK-Hep1 (PSPC1 deficient due to homozygous deletion) and PLC5 (low PSPC1 expression) cells for PSPC1 overexpression and Hep3B (high PSPC1 expression) cells for PSPC1 knockdown in the functional assays with validation of PSPC1 expression by Western blotting (Figure 1A). This evidence concerns the gene PSPC1 and hepatocellular carcinoma.