To confirm FUS and NONO are necessary for IGF1R induction, we silenced FUS and NONO in parental HCC cells of SK-Hep1, Hep3B, and Huh7 without additional expression of PSPC1 and showed the same reduction of IGF1R expression (Figure 6E,F). The gene discussed is FUS; the disease is hepatocellular carcinoma.