We next confirmed in a time kinetic RT-PCR experiment in two additional representative CLL samples (CLL21, 22) our previous observation that gliotoxin inhibited NOTCH2, to a lesser extent NOTCH1, and induced NOTCH3 mRNA expression within 4 h of incubation (Figure 3A) [32]. This evidence concerns the gene NOTCH3 and B-cell chronic lymphocytic leukemia.