Quantification of IHC-stained paraffin-embedded sections revealed that anti-PD-1 plus CCL21-DC lysate vaccine treatment led to the highest CD3 T cell (5- and 2-fold), perforin (4- and 3-fold), and granzyme B (4-and 3-fold) increase and greatest reduction in tumor burden denoted by enhanced caspase-3-stained apoptotic tumor cells (5-and 3-fold) compared with anti-PD-1 and CCL21-DC lysate vaccine monotherapy (Figure 3B). Here, PRF1 is linked to neoplasm.