Purified DCs from the TME of (i) diluent control, (ii) anti-PD-1, (iii) CCL21-DC tumor lysate vaccine, and (iv) anti-PD-1 plus CCL21-DC tumor lysate vaccine were pulsed with MHC Class I K-Ras peptide (LVVVGADGV) or MHC Class II (MTEYKLVVVGADGVG) and co-cultured with splenic CD8 or CD4 T cells of the vaccinated mice at a ratio of 1:5 for 24 h. This evidence concerns the gene CD4 and neoplasm.