To further investigate the relationship between endogenous FAM83G and HSP27 protein levels in other cancerous cells, we estimated the FAM83G mRNA level via qPCR of five noncancerous cell lines, nine NSCLC cell lines with an EGFR mutation, four NSCLC cell lines with a BRAF mutation, 10 NSCLC cell lines with wild-type EGFR/BRAF/KRAS, 11 NSCLC cell lines with a KRAS mutation, and 18 SCLC cell lines with wild-type EGFR/BRAF/KRAS. The gene discussed is HSPB1; the disease is non-small cell lung carcinoma.