To understand why the SQSTM1/p62 protein accumulated in HepG2 cells and was not degraded in Hep3B cells, we investigated whether deptropine could block autophagic flux in hepatoma cells by transfecting cells with the mCherry-green fluorescent protein (GFP)-LC3 tandem reporter plasmid. Here, SQSTM1 is linked to hepatocellular carcinoma.