First, nude mice were subcutaneously inoculated with either LY-10/Vector1 and LY-10/Sirt1 or LY-10/Vector2 and LY-10/Si-Sirt1 (Figure 4A), and then treated with Adriamycin, twice per week, as soon as the tumor became palpable. The gene discussed is SIRT1; the disease is neoplasm.