Amongst the differentially expressed genes, CD274 (PD‐L1) was downregulated in the Cx3cr1‐Rheb1Δ/Δ compared to Rheb1fl/fl GL261 tumours, further highlighting a reduced level of T‐cell inhibition via checkpoint inhibitors in the TME (Fig EV3C). Here, CX3CR1 is linked to neoplasm.