In the present study, multigene testing panels sequencing strategy was used to find the disease‐causing gene of 128 unrelated Chinese families with NSHL, and we identified that WFS1 mutations account for about 3.9% (5/128) of NSHL in Chinese patients negative for the GJB2, SLC26A4, and MT‐RNR1 mutations. This evidence concerns the gene SLC26A4 and nodular sclerosis classical Hodgkin lymphoma.