In the current study, decreased ROS generation in the aortic root and lower serum levels of MDA, ox-LDL, and IL-6 were detected in the berberine-treated ApoE−/− mice, demonstrating the anti-inflammatory and antioxidative properties of berberine, which may be the mechanism by which berberine improves endothelial dysfunction. This evidence concerns the gene IL6 and endothelial dysfunction.