Furthermore, upon transfection of wild-type PTEN into PTEN-deficient glioma cells (U138MG and U87MG), caspase-3 activation, cleavage of PARP-1, and nucleosomal ladder pattern of DNA fragmentation were observed, and these characteristics gradually increased on the 7th and 9th day after transfection. This evidence concerns the gene CASP3 and glioma.