CDKN1A and melanoma: Consistent with the sequencing results, RT-PCR confirmed that GADD45A and CDKN1A were upregulated in the Lj-1-60-treated group, while minichromosome maintenance 3 (MCM3) was downregulated in melanoma cells Sk-Mel-5 and Sk-Mel-28 (Fig. 6a).