Then, we examined the effect of Lj-1-60 on Fyn induced the phosphorylation of Stat3 in vivo, as shown in Fig. 2d, e, both knockdown of Fyn and Lj-1-60 treatment in melanoma cells led to decrease level of phosphorylation of Stat3, indicating that Lj-1-60 is a novel inhibitor targeting Fyn. This evidence concerns the gene STAT3 and melanoma.