After Lj-1-60 treatment, the expression of p-P53, P53, P21, and Bax and the cleavage of PARP was raised, while Bcl-2 had been down-regulated (Fig. 4c), suggesting that Lj-1-60 induced melanoma cell cycle arrested in G2/M phase and apoptosis. Here, CDKN1A is linked to melanoma.