KEGG pathway analysis indicated that multiple signaling pathways were changed in Lj-1-60-treated melanoma cells; among those pathways, DNA replication, cell cycle and P53 signaling pathways were significantly enriched in Sk-Mel-5 cells (Additional file 5: Fig S4B) and Sk-Mel-28 cells (Additional file 5: Fig S4E). Here, TP53 is linked to melanoma.