We found that knocked down of Fyn inhibited the proliferation and induced cell cycle arrest and apoptosis in melanoma Furthermore, we discovered that Lj-1-60 screened out from our in-house lab targeted Fyn and exhibited a strong anti-tumor effect in vivo and in vitro, which is helpful in providing insight into the mechanism of melanoma and developing the anti-tumor drugs. The gene discussed is FYN; the disease is melanoma.